Interactive FAQs Ovulation Question: I had my tubes tied. Five years later I had them untied them and have tried for a baby 4 yrs. I take ovulating tests but never see 2 pink lines? How many days should I test? I want to know why I cant pin point when I ovulate with a kit? I never see the 2 lines? I do every thing right but never a Surge. Tammie S Oregon Answer: The lack of a positive indication on the urine LH test stick usually indicates that you have not ovulated. When to begin testing is based on the length of your menstrual cycles. Subtract about 16 days from the average cycle length and start on that day. So if you have 28 day menstrual cycles, begin testing on cycle day 12. (The first day of heavy flow should be counted as day 1) The kits are accurate about 85% of the time. Rarely, non-specific immune abnormalities can cause false positive or false negative results. Make certain the tests kits are not outdated. I advise my patients to empty their bladder first thing in the morning and then test with the second urine specimen of the day. This can be done 20-30 minutes later and should be done before drinking anything if possible. One clue that you are really ovulating is the basal body temperature chart and change in cervical mucus that occurs with ovulation. The more information you use to monitor your cycle, the better you will be able to take charge of your fertility. In fact, I recommend the book "Taking Charge of Your Fertility" by Toni Wechsler, PhD. to learn more about monitoring your cycles. Dr. Perloe FAQ archives: IVF PCOS Clomiphene Ovulation Induction Injections Age Related Infertility Endometriosis Donor Egg Donor Sperm Male Factor Infertility Fallopian Tube Reanastomosis Tubal Infertility Unexplained Infertility Endometrial Factor Miscellaneous Return to the IVF Homepage This page, and all contents, are Copyright © 2003 by IVF.com, Atlanta, GA, USA.